Thermoresponsive ELP Particles for Improved Cell Expansion

نویسندگان

  • Mariana B Oliveira
  • Laura Martín
  • Wenlong
  • Matilde Alonso
  • J Carlos Rodríguez-Cabello
  • João F Mano
چکیده

Galway, Ireland Thermoresponsive ELP Particles for Improved Cell Expansion Mariana B Oliveira, Laura Martín,Wenlong Song, Matilde Alonso, J Carlos Rodríguez-Cabello, João F Mano, Corresponding Author: [email protected] 3B’s Research Group – Biomaterials, Biodegradables and Biomimetics, Department of Polymer Engineering, University of Minho, AvePark, 4806-909 Taipas, Guimarães, Portugal; IBBInstitute for Biotechnology and Bioengineering, Braga, Portugal. BIOFORGE, University of Valladolid, CIBER-BBN Valladolid, Spain..

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Covalently Adaptable Elastin-Like Protein–Hyaluronic Acid (ELP–HA) Hybrid Hydrogels with Secondary Thermoresponsive Crosslinking for Injectable Stem Cell Delivery

Shear-thinning, self-healing hydrogels are promising vehicles for therapeutic cargo delivery due to their ability to be injected using minimally invasive surgical procedures. An injectable hydrogel using a novel combination of dynamic covalent crosslinking with thermoresponsive engineered proteins is presented. Ex situ at room temperature, rapid gelation occurs through dynamic covalent hydrazon...

متن کامل

Toughening of Thermoresponsive Arrested Networks of Elastin-Like Polypeptides To Engineer Cytocompatible Tissue Scaffolds.

Formulation of tissue engineering or regenerative scaffolds from simple bioactive polymers with tunable structure and mechanics is crucial for the regeneration of complex tissues, and hydrogels from recombinant proteins, such as elastin-like polypeptides (ELPs), are promising platforms to support these applications. The arrested phase separation of ELPs has been shown to yield remarkably stiff,...

متن کامل

Incorporating stimulus-responsive character into filamentous virus assemblies.

Controlling interactions between building blocks, in either guided- or self-assemblies, is becoming increasingly important for the creation of functional materials. We have focused our attention on the well-known model assembly, the filamentous bacteriophage, where our strategy is to selectively alter surface features by focusing on spatially distinct capsid proteins. Towards introducing stimul...

متن کامل

A Highly Elastic and Rapidly Crosslinkable Elastin-Like Polypeptide-Based Hydrogel for Biomedical Applications.

Elastin-like polypeptides (ELPs) are promising for biomedical applications due to their unique thermoresponsive and elastic properties. ELP-based hydrogels have been produced through chemical and enzymatic crosslinking or photocrosslinking of modified ELPs. Herein, a photocrosslinked ELP gel using only canonical amino acids is presented. The inclusion of thiols from a pair of cysteine residues ...

متن کامل

Enhanced uptake of a thermally responsive polypeptide by tumor cells in response to its hyperthermia-mediated phase transition.

Elastin-like polypeptides (ELPs) composed of a VPGXG repeat undergo a reversible phase transition in aqueous solution. They are hydrophilic and soluble in aqueous solution below their transition temperature (T(t)), but they become hydrophobic and aggregate when the temperature is raised above their T(t). In this study, we examine the quantitative uptake of a fluorescence-labeled, thermally resp...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2010